Team:Paris/September 4

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(→PCR Screening of L173 transformants)
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|'''Sample'''
|'''Sample'''
-
|rowspan="3"|1 kb DNA ladder
+
|rowspan="3"|1 kb <br> DNA ladder
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|rowspan="3"|positive PCR control
+
|rowspan="3"|positive PCR <br> control
-
|rowspan="3"|negative PCR control
+
|rowspan="3"|negative PCR <br> control
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|rowspan="3"|negative transformation control clone
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|rowspan="3"|negative transformation <br> control clone
|L173.1
|L173.1
|L173.2
|L173.2
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|rowspan="3"|100 bp DNA ladder
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|rowspan="3"|100 bp <br> DNA ladder
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|-
|'''Expected size'''
|'''Expected size'''

Revision as of 15:59, 4 September 2008

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Contents

PCR Screening of L173 transformants

Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Ligation L173

  • insert: D112 (ES) rbs-TetR
  • vector: D187 (EX) mRFP-LVA-tripart-pSB1A3
Sample positive control
pUC19 		negative control
no DNA 		ligation control
(without insert) 		L173 (3:1 ratio) L173 (4:1 ratio)
Number of colonies many 1 0 2 0

PCR Screening

PCR screening programm

  • elongation tim: 2 min
  • primers used: O18 & O19
  • positive PCR control: S158 (pSB3K3)
  • negative PCR control: no template
Well n° 1 2 3 4 5 6 7
Sample 1 kb
DNA ladder 		positive PCR
control 		negative PCR
control 		negative transformation
control clone 		L173.1 L173.2 100 bp
DNA ladder
Expected size 1894 bp
Measured size

Transformation results

L177 = d134 + d187 (pFlhB-mRFP LVA+)

L175 = d149 + d° (pFliL - ECFP LVA+)

L176 = d149 + d° (pFliL - ECFP LVA-)

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