Team:Paris/September 5

From 2008.igem.org

(Difference between revisions)
(Purification of D112 (rbs-TetR))
(Purification of D112 (rbs-TetR))
Line 10: Line 10:
|'''Measured size'''
|'''Measured size'''
|-
|-
-
|'''pSB1A2'''
+
|'''pSB1A2 (vector)'''
|2079 bp
|2079 bp
|2 kb
|2 kb
|-
|-
-
|'''rbs-TetR'''
+
|'''rbs-TetR (insert)'''
|'''703 bp'''
|'''703 bp'''
|'''0,7 kb'''
|'''0,7 kb'''

Revision as of 15:53, 5 September 2008

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Purification of D112 (rbs-TetR)

Electrophoresis

Expected size Measured size
pSB1A2 (vector) 2079 bp 2 kb
rbs-TetR (insert) 703 bp 0,7 kb

1% agarose gel

Qiaquick Gel Extraction Kit

Sample DNA concentration A260/A280
D112 8 µg/mL 2,31