Team:Paris/September 5

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(Difference between revisions)
(Purification of D112 (rbs-TetR))
(Purification of D112 (rbs-TetR))
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==Electrophoresis and gel extraction==
==Electrophoresis and gel extraction==
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[[Image:KR000300.JPG|thumb|D112 before extraction]]
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[[Image:KR000300.JPG|thumb|D112 before gel excision]]
 +
[[Image:KR000301.JPG|thumb|after gel excision]]
1% agarose gel
1% agarose gel

Revision as of 16:01, 5 September 2008

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Purification of D112 (rbs-TetR)

Electrophoresis and gel extraction

D112 before gel excision
after gel excision

1% agarose gel

Expected size Measured size
pSB1A2 (vector) 2079 bp 2 kb
rbs-TetR (insert) 703 bp 0,7 kb

Qiaquick Gel Extraction Kit

Concentration measurement

Sample DNA concentration A260/A280
D112 8 µg/mL 2,31