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Team:Paris/September 8
From 2008.igem.org
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| + | =Checking our ligases= | ||
| + | Since our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession. | ||
| + | To do so:* we will digest a plasmid with only one restriction enzyme (EcoRI). | ||
| + | * then we will try to ligate it back using our ligases. | ||
| + | ==condition used == | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | ==== | ||
| + | ===njnoinjo=== | ||
Revision as of 13:53, 9 September 2008
Checking our ligasesSince our ligation experiments didn't work during these few days, we presume that our problem came from the ligases. That's why we decided to check the activity of the ligases in our possession. To do so:* we will digest a plasmid with only one restriction enzyme (EcoRI). * then we will try to ligate it back using our ligases. condition used==njnoinjo |
