Team:Paris/September 12

From 2008.igem.org

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(→Checking our ligases (following))
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|D202 <br> 2 µL
|D202 <br> 2 µL
|D202 <br> 3 µL
|D202 <br> 3 µL
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+
|Ligation 1
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+
|Ligation 2
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+
|Ligation 3
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+
|Ligation 4
-
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|Ligation 5
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|Ligation 6
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|Ligation 7
|100 bp ladder
|100 bp ladder
|}
|}

Revision as of 13:49, 12 September 2008

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Checking our ligases (following)

Analysis of the ligation products by electrophosesis

Ligation of the plasmid digested by EcoRI
Ligation of the plasmid digested by BspHI

1% agarose gel

Analysis of the ligation of the plasmid digested by EcoRI

well n° 1 2 3 4 5 6 7 8 9 10 11 12 13
sample 1 kb ladder MP101
0,5 µL 		MP101
1 µL 		D202
2 µL 		D202
3 µL 		Ligation 1 Ligation 2 Ligation 3 Ligation 4 Ligation 5 Ligation 6 Ligation 7 100 bp ladder
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