Revision as of 14:01, 12 September 2008

Checking our ligases (following)

Ligation of the plasmid digested by EcoRI

Ligation of the plasmid digested by BspHI

1% agarose gel

Analysis of the ligation of the plasmid digested by EcoRI No heat inactivation of the ligase

well n°	1	2	3	4	5	6	7	8	9	10	11	12	13
sample	1 kb ladder	MP101	MP101	D202	D202	1	2	3	4	5	6	7	100 bp ladder
purification digestion products						gel extraction			column			no purification
ligase used						L1	L2	L3	L1	L2	L3	L1
amount loaded	10 µL	0,5 µL	1 µL	2 µL	3 µL	10 µL						3 µL	10 µL

@@ Line 30: / Line 30: @@
 |'''sample'''
 |rowspan="3"|1 kb ladder
-|rowspan="3|MP101
+|rowspan="3"|MP101
-|rowspan="3|MP101
+|rowspan="3"|MP101
 |rowspan="3"|D202
 |rowspan="3"|D202
@@ Line 43: / Line 43: @@
 |rowspan="3"|100 bp ladder
 |-
-|'''purification digestion products'''
+|'''purification <br> digestion products'''
 |colspan="3"|gel extraction
 |colspan="3"|column