Team:Paris/September 5

From 2008.igem.org

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=Construction of pFlhB-mRFP-LVA-tripart & pFliL-ECFP-LVA-tripart=
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[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
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*L183 = pFlhB - mRFP LVA+
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*L184 = pFliL - ECFP LVA+
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*L185 = pFliL - ECFP LVA-

Revision as of 18:40, 16 September 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Contents

Purification of yesterday digestion

D112 before gel excision
Digestion n° DNA substrat Digestion by
D112 MP106: S03879 (rbs-TetR) in pSB1A2 EcoRI & SpeI

Electrophoresis and gel extraction

1% agarose gel

Expected size Measured size
pSB1A2 (vector) 2079 bp 2 kb
rbs-TetR (insert) 703 bp 0,7 kb

Qiaquick Gel Extraction Kit

  • elution in 30 µL of EB buffer

Concentration measurement

Sample DNA concentration A260/A280
D112 8 µg/mL 2,31


Construction of pFlhB-mRFP-LVA-tripart & pFliL-ECFP-LVA-tripart

Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png

  • L183 = pFlhB - mRFP LVA+
  • L184 = pFliL - ECFP LVA+
  • L185 = pFliL - ECFP LVA-
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