Team:Paris/July 23

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Revision as of 17:07, 23 July 2008 by Audrey (Talk | contribs)

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Stocks Glycerol

  • 1mL of each culture (with 2 clones) has been added to 1mL of glycerol 40%.
  • For each clone, two stocks glycerol have been done.
  • Stored at -20°C.


MiniPreps

The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 22th.


Name Plasmid Fonction
MP100 B0034 Srtongest RBS
MP101 J23101 Stongest constitutive promoter
MP102 J23109 Weak constitutive promoter
MP103 R0079 lasR activator A
E0030 Part icon reporter.png YFP K
E0040 Part icon reporter.png GFP A
E1010 Part icon reporter.png mRFP K
J23101 Part icon regulatory.png constitutive promoter member family A
J23109 Part icon regulatory.png constitutive promoter member family A
R0040 Part icon regulatory.png tetR repressible promoter A
R0079 Part icon regulatory.png promoter (lasr & PAI regulated) A
pSB1A3 Icon plasmid.png plasmid A
SO3154 Part icon rbs.pngIcon coding.png construction intermediate A
S03879 Part icon rbs.pngIcon coding.png construction intermediate A
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