Team:Paris/July 25

Extraction of the DNA

• Use of Promega's protocol for the extraction of: gel n°1 --> 4 : (D100 --> D110 cl-1 ES))
• Use of Qiagen's protocol for the extraction of: gel n°5 --> 8 : (D110 cl-2 ES --> D120))

• Test of the succeed of the extraction by electrophoresis on 2µl of the parts extracted.

==> conclusion : we don't succeed to detect DNA by spectrophometry or electrophoresis for the samples produced by Qiagen's protocol.

==> we decide to repeat all the samples from the beginning, so we culture O/N at 37°c all the strains usefull for the experiments

MiniPreps

• Use of Promega's protocol on all the clones cultivated on the 24th.
• Preparation of 50µl of minipreps in simple.

list of Minipreps