Team:Paris/August 15

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Contents

1 Transformation of the ligations we did yesterday
2 Digestion
3 Analysis of yesterday PCR screening

Transformation of the ligations we did yesterday

We transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells
standard protocol.

Digestion

Measurement of concentration of minipreps

standard protocol

Digestion	Miniprep used	Concentration (µg/mL)	ratio 260/280
D146	MP148.2	123	1.58
D147	MP153.3	113	1.68

Digestion

Protocol Digestion

Electrophoresis for extraction

standard protocol

Analysis of yesterday PCR screening

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