Team:Paris/August 17

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Analysis of the transformation we did yesterday

Number of colonies and flurorescence

Ligation name Description Antibio Number Colonies observed Fluorescence Comments
Ligations
L150 D105(BV) - D146(BI)
pLas - rbs-TetR-GFP Tripart 		Amp 3 Yes OK
L151 D147(FI) - D125(FV)
rbs-LasR - Double terminator 		Kan 21 No OK
Controls
C1 D105(BV) Amp 150 NO OK
C2 D125(FV) Kan 2 No OK
Positive Control pUC19 Amp 416 (efficiency 4.10^7) No OK

PCR Screening

==> Protocol

Minipreps

Miniprep Name Ligation name Antibio Biobricks Description
MP161.1 L150.1 Amp Part icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - rbs-TetR-GFP tripart
MP161.2 L150.2
MP161.3 L150.3
MP162.1 L151.1 Kan Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-LasR - Double terminator
MP162.2 L151.2
MP162.3 L152.3

Analysis of the other transformation we did yesterday

Evaluation of the number of colonies

Ligation name Number of colonies Autoligation control
L 143 (Kan) 0 0
L 144 (Kan) 0 0
L 145 (Amp) 452 430
L 146 (Amp) ~ 2000 430
L 147 (Amp) ~3500 >5000

PCR Screening

Minipreps

Kok-Phen transformation we did yesterday
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