Team:Paris/August 22

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Contents

1 Analysis of the transformant of FlhDC+promotor
2 Transformation of the ligations we did yesterday
3 Construction for synchronization
- 3.1 Ligations
- 3.2 Transformation

Analysis of the transformant of FlhDC+promotor

Transformation of the ligations we did yesterday

Ligation Name	Description	Antibio
L158	D110(BV) + D131 (BI)	Amp
T L158	D110	Amp
L159	D125(FV)+D109(FI)	Kan
T L159	D125	Kan

Construction for synchronization

Ligations

Ligation Name	Description	Vector Name	Volume vector (µL)	Insert Name	Volume insert(µL)
L162	rbs-lasI + gfp generator	D107 (BV)	10	D163 (BI)	3.46
T L162	autoligation control	D107 (BV)	10
L163	rbs-TetR + gfp generator	D110 (BV)	2.5	D163 (BI)	3.44
T L163	autoligation control	D110 (BV)	2.5

Transformation

Ligation Name	Description	Antibio
L162	D107(BV) + D163 (BI)	Amp
T L162	D107	Amp
L163	D110(BV)+D163(BI)	Amp
T L163	D110	Amp

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