Team:Paris/August 22

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Contents

Analysis of the transformant of FlhDC+promotor

Construction of pFlgA - YFP tripart (+/- LVA)

Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png

Transformation of the ligations we did yesterday

Protocol

Ligation Name Description Antibio
L160 D166(FV) + D132 (FI) Amp
Control L160 D166 Amp
L161 D167(FV) + D1132 (FI) Amp
Control L161 D167 Amp

Construction for synchronization

Transformation of the ligations we did yesterday

Protocol

Ligation Name Description Antibio
L158 D110(BV) + D131 (BI) Amp
T L158 D110 Amp
L159 D125(FV)+D109(FI) Kan
T L159 D125 Kan

Ligation

Protocol

Ligation Name Description Vector Name Volume vector (µL) Insert Name Volume insert(µL)
L162 rbs-lasI + gfp generator
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png 		D107 (BV) 10 D163 (BI) 3.46
T L162 autoligation control D107 (BV) 10
L163 rbs-TetR + gfp generator
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png 		D110 (BV) 2.5 D163 (BI) 3.44
T L163 autoligation control D110 (BV) 2.5

Transformation

Protocol

Ligation Name Description Antibio
L162 D107(BV) + D163 (BI) Amp
T L162 D107 Amp
L163 D110(BV)+D163(BI) Amp
T L163 D110 Amp
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