Team:Paris/July 24

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MiniPreps

The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.

Name	Biobrick	Description
MP116 (=MP1)	J23100
MP117 (=MP2)	J23107
MP118 (=MP3)	B0015
MP119 (=MP4)	I0500
MP120 (=MP5)	B0030
MP121 (=MP6)	E0422
MP122 (=MP8)	E0840

Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name	Miniprep	Biobrick	Enzyme 1	Enzyme 2	Nber tube
D100	MP100	B0034	XbaI	PstI	2
			EcoRI	XbaI	1
			SpeI	PstI	2
D101	MP101	J23101	SpeI	PstI	2
D102	MP102	J23109	SpeI	PstI	2
D103	MP103	R0079	SpeI	PstI	2
D104	MP104	R0040	SpeI	PstI	2
D105	MP105	S03154	SpeI	PstI	1
			XbaI	PstI	2
			EcoRI	SpeI	1
D106	MP106	J23101	SpeI	PstI	1
			XbaI	PstI	2
			EcoRI	SpeI	1
D107	MP107	C0079	EcoRI	SpeI	1
D108	MP108	C0179	EcoRI	SpeI	1
D109	MP109	E0030	EcoRI	SpeI	1
D110	MP110	E0040	EcoRI	SpeI	1
D111	MP111	E1010	EcoRI	SpeI	1
D116	MP116	J23100	SpeI	PstI	2
D117	MP117	J23107	SpeI	PstI	2
D118	MP118	B0015	EcoRI	XbaI	2
D119	MP119	I0500	SpeI	PstI	2
D120	MP120	B0030	SpeI	PstI	2
			EcoRI	XbaI	1
			XbaI	PstI	2
D121	MP121	E0422	XbaI	PstI	3
D122	MP122	E0840	XbaI	PstI	3

Results of digestions : Electrophoresis

Name	BioBrick	Tube N°	Enz 1	Enz 2	Obs	Exp Size Matrix	Exp Size BB	Mea Size Matrix	Mea Size BB
D100	B0034	1	XbaI	PstI	BI	2057 pb	34 pb	not digested	not digested
		2	XbaI	PstI	BI	2057 pb	34 pb	not digested	not digested
		3	EcoRI	XbaI	FV	2076 pb	15 pb	2100 pb	-
		4	SpeI	PstI	BV	2077 pb	14 pb	2000 pb	-
		5	SpeI	PstI	BV	2077 pb	14 pb	2000 pb	-
D101	J23101	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	900 pb
D101	J23101	2	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	900 pb
D102	J23109	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	900 pb
D103	R0079	1	SpeI	PstI	BV	2222 pb	14 pb	2500 pb	-
D103	R0079	2	SpeI	PstI	BV	2222 pb	14 pb	2500 pb	-
D104	R0040	1	SpeI	PstI	BV	2119 pb	14 pb	2500 pb	-
D104	R0040	2	SpeI	PstI	BV	2119 pb	14 pb	2500 pb	-
D105	S03154	1	SpeI	PstI	BV	2750 pb	14 pb	3000 pb	-
		2	XbaI	PstI		2057 pb	707 pb	2000 pb	700 pb
		3	XbaI	PstI		2057 pb	707 pb	2000 pb	700 pb
		4	EcoRI	SpeI		2056 pb	708 pb	2000 pb	700 pb
D106	S03879	1	SpeI	PstI	BV	2768 pb	14 pb	3000 pb	-
		2	XbaI	PstI		2057 pb	725 pb	2000 pb	700 pb
		3	XbaI	PstI		2057 pb	725 pb	2000 pb	700 pb
		4	EcoRI	SpeI		2756 pb	726 pb	2500 pb	700 pb
D107	C0079	1	EcoRI	SpeI	FI	4402 pb	779 pb	6000 pb	1000 pb
D107	C0079	2	XbaI	PstI	BI	4003 pb	778 pb	5000 pb	100 pb
D108	C0179	1	EcoRI	SpeI	FI	4402 pb	746 pb	2500 pb	1000 pb
D108	C0179	2	XbaI	PstI	BI	4403 pb	745 pb	2500 pb	1000 pb
D109	E0030	1	EcoRI	SpeI	FI	3166 pb	746 pb	4000 pb	1000 pb
D109	E0030	2	XbaI	PstI	BI	3167 pb	745 pb	3000 pb	1000 pb
D110	E0040	1	EcoRI	SpeI	FI	2056 pb	743 pb	2000 pb	800 pb
D110	E0040	2	XbaI	PstI	BI	2057 pb	742 pb	2500 pb	1000 pb
D111	E1010	1	EcoRI	SpeI	FI	4402 pb	704 pb	4000 pb	600 pb
D111	E1010	2	XbaI	PstI	BI	4403 pb	703 pb	4500 pb	700 pb
D116	J23100	1	SpeI	PstI	BV	2100 pb	883 pb	Not realised
D117	J23107	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	800 pb
D118	B0015	1	EcoRI	XbaI	FV	3303 pb	15 pb	3000 pb	-
D119	I0500	1	SpeI	PstI	FV	5621 pb	14 pb	6000 - 3000 pb	-
D120	B0030	1	XbaI	PstI	BI	2057 pb	37 pb	not digested	not digested
		2	XbaI	PstI	BI	2057 pb	37 pb	not digested	not digested
		3	EcoRI	XbaI	FV	2079 pb	15 pb	1600 pb	-
		4	SpeI	PstI	BV	2080 pb	14 pb	3000 - 2000 pb	-
		5	SpeI	PstI	BV	2080 pb	14 pb	3000 - 2000 pb	-
D121	E0422	1	XbaI	PstI	FV	2057 pb	939 pb	3000 pb	Not digested
D122	E0840	1	XbaI	PstI	FV	2057 pb	900 pb	2500 pb	Not digested

gel1

gel2

gel3

gel4

gel5

gel6

gel7

gel8