Team:Paris/July 24

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP100 [http://partsregistry.org/Part:BBa_B0034 B0034] Strongest RBS (Efficiency = 1)
MP101 [http://partsregistry.org/Part:BBa_J23101 J23101] Strongest Constitutive promoter
MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] Weak Constitutive promoter
MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] promoter pLas (lasr & PAI regulated)
MP104 [http://partsregistry.org/Part:BBa_R0040 R0040] tetR repressible promoter
MP105 [http://partsregistry.org/Part:BBa_S03154 S03154] B0034(rbs) ->LasI
MP106 [http://partsregistry.org/Part:BBa_S03879 S03879] B0034(rbs) -> TetR
MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] lasR activator with LVA Tag
MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] lasR activator
MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] YFP
MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] GFP
MP111 [http://partsregistry.org/Part:BBa_E1010 E1010] mRFP
MP116 [http://partsregistry.org/Part:BBa_J23100 J23100] Strong constitutive promoter in J61002
MP117 [http://partsregistry.org/Part:BBa_J23107 J23107] Medium constitutive promoter in J61002
MP118 [http://partsregistry.org/Part:BBa_B0015 B0015] Double terminator
MP119 [http://partsregistry.org/Part:BBa_I0500 I0500] AraC pBAD
MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] Strong RBS (Efficiency = 0,6)
MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] ECFP (RBS+LVA+Term)
MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] gfp tri-part; strong rbs

Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2


Results of digestions : Electrophoresis

gel1 [[Image:| gel1|200px]] gel2 [[Image:| gel2|200px]] gel3 [[Image:| gel3|200px]] gel4 [[Image:| gel4|200px]]


gel5 [[Image:‎| gel5|200px]] gel6 [[Image:‎| gel6|200px]] gel7 [[Image:| gel7|100px]] gel8 [[Image:|100px]]


Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB Gel Band
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb
2
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb
2
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb
2
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb
2 XbaI PstI BI 2057 pb 707 pb
3
4 EcoRI SpeI FI 2056 pb 708 pb
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb
2 XbaI PstI BI 2057 pb 725 pb
3
4 EcoRI SpeI FI 2756 pb 726 pb
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb
2 XbaI PstI BI 4003 pb 778 pb
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb
2 XbaI PstI BI 4403 pb 745 pb
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb
2 XbaI PstI BI 3167 pb 745 pb
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb
2 XbaI PstI BI 2057 pb 742 pb
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb
2 XbaI PstI BI 4403 pb 703 pb
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb
2
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb
2
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb
2
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb
2
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb
2
3 EcoRI XbaI FV 2079 pb 15 pb
4 SpeI PstI BV 2080 pb 14 pb
5
D121 E0422 1 XbaI PstI BI 2057 pb 939 pb
D122 E0840 1 XbaI PstI BI 2057 pb 900 pb
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