Team:Paris/July 30

From 2008.igem.org

Revision as of 16:52, 4 August 2008 by KP (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Analysis of yesterday DNA digestion

The digested DNA of yesterday was analysed one more time by electrophoresis on a 0.8% agarose gel (about 30 minutes at 100 W). The ladder used was the 1 kb DNA ladder (New England Biolabs). 5 µL of each sample with 1 µL of loading dye were loaded.

D101	B0034	1	EcoRI	XbaI	FV	2076 bp	2000 bp	6	10
Name	BioBrick	Tube N°	Enz 1	Enz 2	Obs	Exp Size	Mea Size	Conc (ng/µl)	Band
D102	B0034	1	SpeI	PstI	BV	2077 bp	2000 bp	8	11
D105	R0079	1	SpeI	PstI	BV	2222 bp	2000 bp	6	13
D106	R0040	1	SpeI	PstI	BV	2119 bp	2000 bp	6	12
D108	S03154	1	XbaI	PstI	BI	707 bp		0 (failed)	14
D111	S03879	1	XbaI	PstI	BI	725 bp	600 bp	2	15
D115	C0179	2	EcoRI	SpeI	FI	746 pb		0 (failed)	4 & 5
D116	C0179	2	XbaI	PstI	BI	745 pb			2 & 3
D117	E0030	1	EcoRI	SpeI	FI	746 pb			16
D128	B0030	2	EcoRI	XbaI	FV	2079 pb			6 & 7
D129	B0030	2	SpeI	PstI	BV	2080 pb			8 & 9

Results : Each of the samples was succesfully digested and purified except for the sample D108. It seems that the QIAprep columms (from the QIAGEN Minipreps kit) can be used instead of the QIAquick columms (for DNA Gel Extraction).