| Ligation name
| Nb of colonies
| Description
|
| L100
| 0
| rbs-TetR <-> ECFP (RBS+LVA+Term)
|
| L101
| (1)
| rbs-TetR <-> GFP tripart+strong rbs
|
| L102
| +/- 100
| strong RBS (0,6)<-> YFP
|
| L103
| a lot ++
| strong RBS (0,6)<-> GFP
|
| L104
| a lot ++
| strong RBS (0,6)<-> LasR activator (+LVA)
|
| L105
| a lot ++
| strong constitutive promoter <-> ECFP
| 5+8
|
| L106
| a lot ++
| strong constitutive promoter <-> GFP tripart
|
| L107
| a lot ++
| strongest constitutive promoter <-> ECFP
|
| L108
| +/- 100 (2 dishes!?)
| strongest constitutive promoter <-> GFP tripart
|
| L109
| +/- 30 or a lot ++ (2 dishes !?)
| medium constitutive promoter <-> ECFP
|
| L110
| a lot ++
| medium constitutive promoter <-> gfp tripart
|
| L111
| a lot ++
| weak constitutive promoter <-> ECFP
|
| L112
| a lot ++
| weak constitutive promoter <-> gfp tripart
|
| L113
| 1
| Arac pBAD <-> ECFP
|
| L114
| 2
| Arac pBAD <-> gfp tripart
|
| L115
| a lot ++
| pLas <-> ECFP
|
| L116
| a lot ++
| pLas <-> gfp tripart
|
| L117
| +/- 30
| Double terminator <-> YFP
|
| L118
| +/- 30
| Double terminator <-> mRFP
|
| L119
| +/- 30
| Double terminator <-> LasR activator (+LVA)
|
| L120
| a lot ++
| tetR repressible promoter <-> ECFP
|
| L121
| a lot ++
| tetR repressible promoter <-> gfp tripart
|
| L122
| 0
| B0034rbs-LasI <-> ECFP
|
| L123
| 1
| B0034rbs-LasI <-> gfp tripart
|
| L124
| a lot ++
| strongest RBS (1) <-> GFP
|
| L125
| a lot ++
| strongest RBS (1) <-> YFP
|
| L126
| no dish found!!!
| strongest RBS (1) <-> activator (+LVA)
|
| L127
| no dish found!!!
| Double terminator <-> GFP
|
| C1
| 0
| D110
|
| C2
| 5
| D129
|
| C3
| +/- 100
| D123
|
| C4
| +/- 20
| D103
|
| C5
| +/- 100
| Double terminator <-> GFP
|
| C6
| +/- 100
| Double terminator <-> GFP
|
| C7
| 0
| Double terminator <-> GFP
|
| C8
| +/- 20
| Double terminator <-> GFP
|
| C9
| 0
| Double terminator <-> GFP
|
| C10
| 0
| Double terminator <-> GFP
|
| C11
| 0
| Double terminator <-> GFP
|
| C12
| +/- 10
| Double terminator <-> GFP
|
| Positive control
| 155 (transformation efficiency:1.5*10^7/ug)
| -
|
"
