Team:Paris/August 12

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Contents

1 Glycerol Stocks
2 Minipreps: Plasmid extraction
3 Results of the transformations we did yesterday
4 Concetration of the MiniPreps

Glycerol Stocks

Protocol (see #2)

List of Stocks

Strain	Ligation	Parts
S143.1	L128.1	J61002-pFlgA D136 (FV) - D132 (FI)
S143.2	L128.2
S143.3	L128.3
S143.4	L128.4
S144.1	L129.1	J61002-pFlgB D136 (FV) - D133 (FI)
S144.2	L129.2
S144.6	L129.6
S144.7	L129.7
S145.1	L130.1	J61002-pFlhB D136 (FV) - D134 (FI)
S145.2	L130.2
S145.7	L130.7
S145.8	L130.8

Minipreps: Plasmid extraction

Protocol (see #3) Experiments done by QIAcube

List of Minipreps

Name	Ligation	Biobricks	Description
MP144	L128.1	pFlgA-RFP D132 (FI) - D136 (FV)
MP145	L128.2	pFlgA-RFP D132 (FI) - D136 (FV)
MP146	L128.3	pFlgA-RFP D132 (FI) - D136 (FV)
MP147	L128.4	pFlgA-RFP D132 (FI) - D136 (FV)
MP148	L129.1	pFlgB-RFP D133 (FI) - D136 (FV)
MP149	L129.2	pFlgB-RFP D133 (FI) - D136 (FV)
MP150	L129.6	pFlgB-RFP D133 (FI) - D136 (FV)
MP151	L129.7	pFlgB-RFP D133 (FI) - D136 (FV)
MP152	L130.1	pFlhB-RFP D134 (FI) - D136 (FV)
MP153	L130.2	pFlhB-RFP D134 (FI) - D136 (FV)
MP154	L130.7	pFlhB-RFP D134 (FI) - D136 (FV)
MP155	L130.8	pFlhB-RFP D134 (FI) - D136 (FV)

Results of the transformations we did yesterday

Concetration of the MiniPreps

Miniprep	Concentration (µg/mL)	Ratio 260/280
MP144	127	1.67
MP145	166	1.66
MP146	163	1.57
MP147	154	1.63
MP148	136	1.67
MP149	151	1.63
MP150	163	1.67
MP151	188	1.57
MP152	298	1.70
MP153	142	1.64
MP154	170	1.52
MP155	143	1.55

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