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Project
Analysis
Construction
Characterization
Perspectives
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Ligation L173 (
rbs-TetR
in
mRFP-LVA-tripart
-pSB1A3)
Samples used
Name
Digestion number
Digested by
DNA concentration
rbs-TetR
D112
EcoRI & SpeI (insert)
~20 µg/mL
mRFP-LVA-tripart
D187
EcoRI & XbaI (vector)
16 µg/mL
Reaction mixtures
Control
Sample
3:1
(insert:vector)
ratio
4:1
(insert:vector)
ratio
D112
(insert)
0 µL
1,4 µL
1,9 µL
D187
(vector)
2,5 µL
2,5 µL
2,5 µL
T4 DNA ligase 10X
buffer
2 µL
2 µL
2 µL
T4 DNA
ligase
1 µL
1 µL
1 µL
water
14,5 µL
13,1 µL
12,6 µL
Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).
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