Team:Paris/August 28

From 2008.igem.org

Revision as of 19:00, 9 September 2008 by Fanny.c (Talk | contribs)

← Yesterday

↓ Calendar ↑

Tomorrow →

Contents

Transformation of OmpR*

Cloning of EnvZ*

The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product.

Digestion

Digestion of EnvZ* and lasR-pSB1A2
Excision of the band corresponding to pSB1A2
Digestion n° Name volume of DNA digested total volume reaction enzymes used
D159 PCR147 (EnvZ*) 3 µL 30 µL XbaI & PstI
D116 MP108 (lasR-pSB1A2) 2 µL 30 µL XbaI & PstI


Purification

  • EnvZ*: directly by the Qiagen kit
  • pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit


Digestion

Protocol

We did again D110 and D125.

Retrieved from "http://2008.igem.org/Team:Paris/August_28"