Team:Paris/August 28

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Contents

1 Transformation of OmpR*
2 Cloning of EnvZ*
- 2.1 Digestion
3 Freezer empty
- 3.1 Repeat of Digestion

Transformation of OmpR*

Cloning of EnvZ*

The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product.

Digestion

Digestion of EnvZ* and lasR-pSB1A2

Digestion n°	Name	volume of DNA digested	total volume reaction	enzymes used
D159	PCR147 (EnvZ*)	3 µL	30 µL	XbaI & PstI
D116	MP108 (lasR-pSB1A2)	2 µL	30 µL	XbaI & PstI

Purification

EnvZ*: directly by the Qiagen kit
pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit

Freezer empty

Repeat of Digestion

We did again D110 and D125.

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