Checking our ligases (following)

Analysis of the ligation products by electrophosesis

Ligation of the plasmid digested by EcoRI

Ligation of the plasmid digested by BspHI

1% agarose gel

Analysis of the ligation of the plasmid digested by EcoRI No heat inactivation of the ligase

well n°	1	2	3	4	5	6	7	8	9	10	11	12	13
sample	1 kb ladder	MP101	MP101	D202	D202	1	2	3	4	5	6	7	100 bp ladder
purification digestion products		gel extraction				column			no purification
ligase used		L1	L2			L3	L1	L2	L3	L1
amount loaded	10 µL	0,5 µL	1 µL		2 µL	3 µL	10 µL						3 µL	10 µL