Team:Paris/July 11

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Cultures

The strains stocked at -80°C were taken out and spread (streaks) on LB plates (without antibiotic). Plates have been put in the steam room at 37°C at 15h45

Code	Strain
DW 27	MG1655 ΔfliA::Cm
NEC 283	MG 1655 ΔfliA::FRT
NEC 284	MG 1655 ΔflhD::FRT
DW 26	MG 1655 ΔflgM::Cm
NEC 285	MG 1655 ΔflgM::FRT
DW 35	JW K1879 ΔmotA::Kan
NEC 288	MG 1655 ΔmotA::FRT
DW 37	JW K1068 ΔflgJ::Kan
NEC 287	MG 1655 ΔflgJ::FRT
DW 24	MG 1655 ΔfliC::Cm
NEC 286	MG 1655 ΔfliC::FRT

Manipulations

Culture of DH5ɑ in 5mL of LB
Preparation of 250mL of CaCl₂ 0,1M
Transfer of the equipment iGEM 07 on the sixth floor