Team:Paris/July 18

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Contents

Analysis of the Electrophoresis

Biobricks Clone Expected size Measured size
E1010 1 5.106 kb ~6-8 kb
E1010 2 5.106 kb ~6-8 kb
C0079 1 5.181 kb ~6-8 kb
C0079 2 5.181 kb ~6-8 kb
C0079 3 5.181 kb ~6-8 kb
J23101 : problem !!! 1 3.037 kb ~6-8 kb
J23101 : problem !!! 2 3.037 kb ~6-8 kb
J23101 : problem !!! 3 3.037 kb ~6-8 kb
B0034 2 2.091 kb ~2 kb
B0034 X 2.091 kb ~2 kb
B0034 : problem !!! Y 2.091 kb ~5 kb
E0040 1 2.799 kb ~2.5-3 kb
E0040 2 2.799 kb ~2.5-3 kb
R0040 ? 2.133 kb ~2 kb

Culture of chosen clones carrying BioBricks

Chosen Clones

Biobricks Antibio Chosen Clones
B0034 A 1, 2 & 3
R0079 A 3, 4 & 6
C0079 K 1, 2 & 3
C0179 A 1, 2 & 7
J23101 A 1, 2 & 3
J23109 A 1, 2 & 5
R0040 A 1 & 2
pSB1A3 A 1, 2 & 3
E1010 K 1, 2 & 3
E0040 A 1 & 2
E0030 K 1, 2 & 3

Overnight culture of selected clones

Culture of the selected clones 5 mL of LB media containing the apropriate antibiotic. Incubation at 37°C overnight.

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