Team:Paris/July 21

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Contents

1 Quantitative analysis of DNA
- 1.1 Electrophoresis
  - 1.1.1 Gel 1
  - 1.1.2 Gel 2
- 1.2 Measurement of DNA concentration

Quantitative analysis of DNA

Electrophoresis

Gel : 0.8 % agarose
10µL Quick-Load 1kb DNA Ladder
2µL Bromophenol Blue 5X + 2µL DNA

Gel 1

Band	Biobricks	Clone	Expected size	Measured size
1	Ladder 1 kb
2	B0034 : Problem !!!	1	2.091 kb	~ 1.6 kb
3	B0034 : Problem !!!	2	2.091 kb	~ 1.6 kb
4	B0034 : Problem !!!	3	2.091 kb	~ 3 kb
5	R0040 : Problem !!!	1	2.133 kb	~ 1.7 kb
6	R0040 : Problem !!!	2	2.133 kb	~ 1.7 kb

Gel 2

Gel : 0.8 % agarose
10µL Quick-Load 1kb DNA Ladder
2µL + 2µL DNA

Band	Biobricks	Clone	Expected size	Measured size
1	Ladder 1 kb
2	J23109 : Problem !!!	1	3.037 kb	~ 1.7 kb
3	J23109 : Problem !!!	2	3.037 kb	~ 5 kb
4	J23109 : Problem !!!	3	3.037 kb	~ 1.7 kb
5	C0179 : Problem !!!	1	2.802 kb	> 10 kb
6	C0179 : Problem !!!	2	2.802 kb	~ 5 kb
7	C0179 : Problem !!!	3	2.802 kb	~ 5 kb

Measurement of DNA concentration

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