Team:Paris/July 21

From 2008.igem.org

Revision as of 15:29, 21 July 2008 by Cyprien (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

← Yesterday

↓ Calendar ↑

Tomorrow →

Contents

Quantitative analysis of DNA

Electrophoresis

Gel : 0.8 % agarose
10 µL Quick-Load 1 kb DNA Ladder
2 µL Bromophenol Blue 5X + 2 µL DNA

Gel 1

KR000016.jpg


Band Biobricks Clone Expected size Measured size
1 Ladder 1 kb      
2 B0034 : Problem !!! 1 2.091 kb ~ 1.6 kb
3 B0034 : Problem !!! 2 2.091 kb ~ 1.6 kb
4 B0034 : Problem !!! 3 2.091 kb ~ 3 kb
5 R0040 : Problem !!! 1 2.133 kb ~ 1.7 kb
6 R0040 : Problem !!! 2 2.133 kb ~ 1.7 kb

Gel 2

KR000018.jpg
Band Biobricks Clone Expected size Measured size
1 Ladder 1 kb      
2 J23109 : Problem !!! 1 3.037 kb ~ 1.7 kb
3 J23109 : Problem !!! 2 3.037 kb ~ 5 kb
4 J23109 : Problem !!! 3 3.037 kb ~ 1.7 kb
5 C0179 : Problem !!! 1 2.802 kb > 10 kb
6 C0179 : Problem !!! 2 2.802 kb ~ 5 kb
7 C0179 : Problem !!! 3 2.802 kb ~ 5 kb

Measurement of DNA concentration
Retrieved from "http://2008.igem.org/Team:Paris/July_21"