Team:Paris/July 25

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 24th.
Name Biobrick Description
MP100 B0034
MP116 J23100
MP120 B0030
MP121 E0422
MP122 E0840



Digestion

Digestion Mix

10µl of Miniprep
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D116 MP116 J23100 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 1 !
D122 MP122 E0840 XbaI PstI 1 !


Results of digestions : Electrophoresis

Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb
2
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb
2
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb
2
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb
2 XbaI PstI 2057 pb 707 pb
3
4 EcoRI SpeI 2056 pb 708 pb
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb
2 XbaI PstI 2057 pb 725 pb
3
4 EcoRI SpeI 2756 pb 726 pb
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb
2 XbaI PstI BI 4003 pb 778 pb
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb
2 XbaI PstI BI 4403 pb 745 pb
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb
2 XbaI PstI BI 3167 pb 745 pb
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb
2 XbaI PstI BI 2057 pb 742 pb
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb
2 XbaI PstI BI 4403 pb 703 pb
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb
2
3 EcoRI XbaI FV 2079 pb 15 pb
4 SpeI PstI BV 2080 pb 14 pb
5
D121 E0422 1 XbaI PstI FV 2057 pb 939 pb
D122 E0840 1 XbaI PstI FV 2057 pb 900 pb
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