Team:Paris/July 28

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Extraction of the DNA

Results of the extraction : Electrophoresis

conditions :

  • 10µl of ladder 1 kb
  • 2µl of extraction added with 2µl of loading Dye 6x
  • migration ~30min at 100W
  • Gel 1, 2, 3, 4, 5 = 0.8%
  • Gel 6 = 1,5%


gel1 gel1 gel2 gel2 gel3 gel3



Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Mea Size Conc (ng/µl) Gel Band
D100 B0034 1 XbaI PstI BI 2057 pb
2
D101 B0034 3 EcoRI XbaI FV 2076 pb - - 1 2
D102 B0034 4 SpeI PstI BV 2077 pb - - 1 3
5 - 1 4
D103 J23101 1 SpeI PstI BV 2100 pb - - 1 5
2 - 1 6
D104 J23109 1 SpeI PstI BV 2100 pb 1 7
2 1 8
D105 R0079 1 SpeI PstI BV 2222 pb 2 2
2 2 3
D106 R0040 1 SpeI PstI BV 2119 pb 2 4
2 2 5
D107 S03154 1 SpeI PstI BV 2750 pb 2 6
D108 S03154 2 XbaI PstI BI 707 pb 2 7
3 2 8
D109 S03154 4 EcoRI SpeI FI 708 pb 3 2
D110 S03879 1 SpeI PstI BV 2768 pb 3 3
D111 S03879 2 XbaI PstI BI 725 pb 3 4
3 3 5
D112 S03879 4 EcoRI SpeI FI 726 pb 3 6
D113 C0079 1 EcoRI SpeI FI 779 pb 3 7
D114 C0079 2 XbaI PstI BI 778 pb 3 8
D115 C0179 1 EcoRI SpeI FI 746 pb
D116 C0179 2 XbaI PstI BI 745 pb
D117 E0030 1 EcoRI SpeI FI 746 pb
D118 E0030 2 XbaI PstI BI 745 pb
D119 E0040 1 EcoRI SpeI FI 743 pb
D120 E0040 2 XbaI PstI BI 742 pb
D121 E1010 1 EcoRI SpeI FI 704 pb
D122 E1010 2 XbaI PstI BI 703 pb
D123 J23100 1 SpeI PstI BV 2100 pb
2
D124 J23107 1 SpeI PstI BV 2100 pb
2
D124 J23107 1 EcoRI XbaI FV 3303 pb
2
D126 I0500 1 SpeI PstI FV 5621 pb
2
D127 B0030 1 XbaI PstI BI 37 pb
2
D128 B0030 3 EcoRI XbaI FV 2079 pb
D129 B0030 4 SpeI PstI BV 2080 pb
5
D130 E0422 1 XbaI PstI BI 939 pb
2
3
D131 E0840 1 XbaI PstI BI 900 pb
2
3


==> conclusion :

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