| Ligation name
| Nb of colonies
| Description
| Volume gel extraction
|
| L100
| 0
| rbs-TetR <-> ECFP (RBS+LVA+Term)
| 3+6
|
| L101
| (1)
| rbs-TetR <-> GFP tripart+strong rbs
| 3+6
|
| L102
| +/- 100
| strong RBS (0,6)<-> YFP
| 5+10
|
| L103
| a lot ++
| strong RBS (0,6)<-> GFP
| 5+5
|
| L104
| a lot ++
| strong RBS (0,6)<-> LasR activator (+LVA)
| 5+4
|
| L105
| a lot ++
| strong constitutive promoter <-> ECFP
| 5+8
|
| L106
| a lot ++
| strong constitutive promoter <-> GFP tripart
| 5+8
|
| L107
| a lot ++
| strongest constitutive promoter <-> ECFP
| 5+8
|
| L108
| +/- 100 (2 dishes!?)
| strongest constitutive promoter <-> GFP tripart
| 5+8
|
| L109
| +/- 30 or a lot ++ (2 dishes !?)
| medium constitutive promoter <-> ECFP
| 4+7
|
| L110
| a lot ++
| medium constitutive promoter <-> gfp tripart
| 4+7
|
| L111
| a lot ++
| weak constitutive promoter <-> ECFP
| 3+5
|
| L112
| a lot ++
| weak constitutive promoter <-> gfp tripart
| 3+5
|
| L113
| 1
| Arac pBAD <-> ECFP
| 5+2.5
|
| L114
| 2
| Arac pBAD <-> gfp tripart
| 5+2.5
|
| L115
| a lot ++
| pLas <-> ECFP
| 7+11
|
| L116
| a lot ++
| pLas <-> gfp tripart
| 7+11
|
| L117
| +/- 30
| Double terminator <-> YFP
| 4+3
|
| L118
| +/- 30
| Double terminator <-> mRFP
| 4+2
|
| L119
| +/- 30
| Double terminator <-> LasR activator (+LVA)
| 4+2
|
| L120
| a lot ++
| tetR repressible promoter <-> ECFP
| 6+7.5
|
| L121
| a lot ++
| tetR repressible promoter <-> gfp tripart
| 6+7.5
|
| L122
| 0
| B0034rbs-LasI <-> ECFP
| 7+3.5
|
| L123
| 1
| B0034rbs-LasI <-> gfp tripart
| 7+3.5
|
| L124
| a lot ++
| strongest RBS (1) <-> GFP
| 6+5
|
| L125
| a lot ++
| strongest RBS (1) <-> YFP
| 6+10
|
| L126
| no dish found!!!
| strongest RBS (1) <-> activator (+LVA)
| 6+4
|
| L127
| no dish found!!!
| Double terminator <-> GFP
| 6+3
|
| Controls
|
|
|
|
| C1
| 0
| D110
| 3
|
| C2
| 5
| D129
| 5
|
| C3
| +/- 100
| D123
| 5
|
| C4
| +/- 20
| D103
| 5
|
| C5
| +/- 100
| Double terminator <-> GFP
| 4
|
| C6
| +/- 100
| Double terminator <-> GFP
| 3
|
| C7
| 0
| Double terminator <-> GFP
| 5
|
| C8
| +/- 20
| Double terminator <-> GFP
| 7
|
| C9
| 0
| Double terminator <-> GFP
| 4
|
| C10
| 0
| Double terminator <-> GFP
| 6
|
| C11
| 0
| Double terminator <-> GFP
| 7
|
| C12
| +/- 10
| Double terminator <-> GFP
| 6
|
| Positive control
| 155 (transformation efficiency:1.5*10^7/ug)
| ???
|
|
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