Team:Paris/August 2

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Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence
puc19 positive control A CFU
L104 Strong rbs - lasR activator


D129 (BV) - D114 (BI)

L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

L108

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

L115

pLas - ECFP
D105 (BV) - D130 (BI)

L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

L117

Double Terminator - yfp
D125 (FV) - D117 (FI)

L118

Double Terminator - rfp
D125 (FV) - D121 (FI)

L119

Double Terminator - lasR activator + LVA
D125 (FV) - D113 (FI)

L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)


L100 A 0 CFU 0 CFU
L113 K few
L114 K medium
L120 A few
L122 A medium
L123 A 0 CFU
L126 A a lot