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Revision as of 16:17, 11 August 2008 by
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Project
Analysis
Construction
Characterization
Perspectives
← Yesterday
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June
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October
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Culture of ligation transformants
4 clones of each transformation were cultured in
7,5 mL LB + ampicilline
. The colonies picked up were the rest of those already picked up from the transformation plate (for PCR screening).
37°C overnight
Ligation
L128
L129
L130
Name
pFlgA
pFlgB
pFlhB
Clone N°
1
2
3
4
1
2
6
7
1
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7
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Red fluorescence
yes
yes
no
no
yes
yes
no
no
yes
no
no
no