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Revision as of 18:09, 11 August 2008 by
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Project
Analysis
Construction
Characterization
Perspectives
← Yesterday
↓ Calendar ↑
Tomorrow →
June
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July
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August
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September
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October
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November
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Contents
1
Transformation
2
Digestion
3
PCR
4
Culture of ligation transformants
Transformation
Protocol
: (see #
13
)
Digestion
PCR
Culture of ligation transformants
4 clones of each transformation were cultured in
7,5 mL LB + ampicilline
. The colonies picked up were the rest of those already picked up from the transformation plate (for the PCR screening of August 8).
37°C overnight
Ligation
L128
L129
L130
Name
pFlgA
pFlgB
pFlhB
Clone N°
1
2
3
4
1
2
6
7
1
2
7
8
Red fluorescence
yes
yes
no
no
yes
yes
no
no
yes
no
no
no
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