Team:Paris/August 12

From 2008.igem.org

Revision as of 17:22, 12 August 2008 by Audrey (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

↓ Calendar ↑

Contents

1 Minipreps: Plasmid extraction
2 Glycerol Stocks
3 Results of the transformations we did yesterday
4 Concentration of the MiniPreps

Minipreps: Plasmid extraction

Protocol (see # 3) Experiments done by QIAcube

List of Minipreps

Name	Ligation	Biobricks	Description
MP144	L128.1	pFlgA-RFP D132 (FI) - D136 (FV)
	L128.2	pFlgA-RFP D132 (FI) - D136 (FV)
	L128.3	pFlgA-RFP D132 (FI) - D136 (FV)
	L128.4	pFlgA-RFP D132 (FI) - D136 (FV)
MP145	L129.1	pFlgB-RFP D133 (FI) - D136 (FV)
	L129.2	pFlgB-RFP D133 (FI) - D136 (FV)
	L129.6	pFlgB-RFP D133 (FI) - D136 (FV)
	L129.7	pFlgB-RFP D133 (FI) - D136 (FV)
MP146	L130.1	pFlhB-RFP D134 (FI) - D136 (FV)
	L130.2	pFlhB-RFP D134 (FI) - D136 (FV)
	L130.7	pFlhB-RFP D134 (FI) - D136 (FV)
	L130.8	pFlhB-RFP D134 (FI) - D136 (FV)

Glycerol Stocks

Protocol (see #2)

List of Stocks

Strain	Ligation	Biobricks	Description
S143.1	L128.1	pFlgA-RFP D132 (FI) - D136 (FV)
S143.2	L128.2
S143.3	L128.3
S143.4	L128.4
S144.1	L129.1	pFlgB-RFP D133 (FI) - D136 (FV)
S144.2	L129.2
S144.6	L129.6
S144.7	L129.7
S145.1	L130.1	pFlhB-RFP D134 (FI) - D136 (FV)
S145.2	L130.2
S145.7	L130.7
S145.8	L130.8

Results of the transformations we did yesterday

Concentration of the MiniPreps

Miniprep	Concentration (µg/mL)	Ratio 260/280
MP144	127	1.67
MP145	166	1.66
MP146	163	1.57
MP147	154	1.63
MP148	136	1.67
MP149	151	1.63
MP150	163	1.67
MP151	188	1.57
MP152	298	1.70
MP153	142	1.64
MP154	170	1.52
MP155	143	1.55

Retrieved from "http://2008.igem.org/Team:Paris/August_12"