Team:Paris/August 12

Minipreps: Plasmid extraction

• Protocol (see # 3) Experiments done by QIAcube

• List of Minipreps

Name	Ligation	Biobricks	Description
MP144	L128.1	pFlgA-RFP D132 (FI) - D136 (FV)
	L128.2	pFlgA-RFP D132 (FI) - D136 (FV)
	L128.3	pFlgA-RFP D132 (FI) - D136 (FV)
	L128.4	pFlgA-RFP D132 (FI) - D136 (FV)
MP145	L129.1	pFlgB-RFP D133 (FI) - D136 (FV)
	L129.2	pFlgB-RFP D133 (FI) - D136 (FV)
	L129.6	pFlgB-RFP D133 (FI) - D136 (FV)
	L129.7	pFlgB-RFP D133 (FI) - D136 (FV)
MP146	L130.1	pFlhB-RFP D134 (FI) - D136 (FV)
	L130.2	pFlhB-RFP D134 (FI) - D136 (FV)
	L130.7	pFlhB-RFP D134 (FI) - D136 (FV)
	L130.8	pFlhB-RFP D134 (FI) - D136 (FV)

Glycerol Stocks

• Protocol (see #2)

• List of Stocks

Strain	Ligation	Biobricks	Description
S143.1	L128.1	pFlgA-RFP D132 (FI) - D136 (FV)
S143.2	L128.2
S143.3	L128.3
S143.4	L128.4
S144.1	L129.1	pFlgB-RFP D133 (FI) - D136 (FV)
S144.2	L129.2
S144.6	L129.6
S144.7	L129.7
S145.1	L130.1	pFlhB-RFP D134 (FI) - D136 (FV)
S145.2	L130.2
S145.7	L130.7
S145.8	L130.8

Results of the transformations we did yesterday

Concentration of the MiniPreps

New PCR screening with the right pimers

Transformants with pFlgA, pFlgB and pFlhB cloned into J61002 are analysed by PCR but this time with the right primers (O18 and O19).

• Template : bacteria from glycerol stock.
• positive control: J61002 (with ptet-mRFP)
• negative control: no template

Electrophoresis

• 1% agarose gel
• 10 µL of each sample loaded