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Revision as of 14:09, 17 August 2008 by
Audrey
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Project
Analysis
Construction
Characterization
Perspectives
← Yesterday
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June
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July
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August
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September
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October
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November
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Analysis of the transformation we did
yesterday
Ligation name
Description
Antibio
Number Colonies observed
Fluorescence
Comments
Ligations
L150
D105(BV) - D146(BI)
pLas - rbs-TetR-GFP Tripart
Amp
3
Yes
OK
L151
D147(FI) - D125(FV)
rbs-LasR - Double terminator
Kan
21
No
OK
Controls
C1
D105(BV)
Amp
150
NO
OK
C2
D125(FV)
Kan
2
No
OK
Positive Control
pUC19
Amp
416 (efficiency 4.10^7)
No
OK
PCR Screening
Protocol
Minipreps
Miniprep Name
Ligation name
Antibio'
Biobricks
Description
MP161.1
L150.1
Amp
pLas - rbs-TetR-GFP tripart
MP161.2
L150.2
MP161.3
L150.3
MP162.1
L151.1
Kan
rbs-LasR - Double terminator
MP162.2
L151.2
MP162.3
L152.3
"
