Team:Paris/August 16

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Analysis of the transformation we did yesterday

L143, L144, T1 and T2 showed no colonies. The positive control with pUC19 worked well.
We suppose that the ligation did not work. We will do it again today.

Ligation

Cleaning of the DNA after the digestion

We used the QIAcube to wash the DNA, following the standard protocol.

Measure of DNA concentration of the digestion products

List of ligations

Protocol

Transformation

Transformation protocol

Name	Description	Antibio
Ligations
L150	D105(BV) - D146(BI) pLas - strongest rbs-TetR-GFP tripart	Amp
L151	D147(FI) - D125 (FV) strongest rbs-LasR activator with LVA - Double terminator	Kan
Controls
C1	D105	Amp
C2	D125	Kan
Positive Control	pUC19	Amp