Team:Paris/August 24

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Construction for synchronization

Gel extraction

Gel 1 (no image sorry, we forgot to save it!)

Well	1	2	3	4	5	6	7	8	9	10	11	12
Sample	1kb ladder	MP105.1	no sample	D107	no sample	D109	no sample	MP106	no sample	D110	no sample	100pb ladder
Expected size (pb)	-	2764	-	2750 14	-	2056 708	-	2782	-	2768 14	-	-
Measured size (pb)	-	2800	-	2750	-	2000 700	-	2800	-	2750	-	-

Gel 2

Well	1	2	3	4	5	6	7	8	9	10	11	12
Sample	no sample	1kb ladder	MP118.1	no sample	D125	no sample	MP143	no sample	D163	no sample	100pb ladder	no sample
Expected size (pb)	-	-	3318	-	3303 15	-	3322	-	2432 890	-	-	-
Measured size (pb)	-	-	2900	-	3300	-	2900	-	2500 900	-	-	-

Ligation

Ligation Name	Vector Name	Volume Vector (µL)	Insert	Volume Insert (µL)
L159	D125	6	D109	3.86
L162	D107	5	D163	5.3
L163	D110	7.5	D163	5.32
Control L159	D125	6	-	-
Control L162	D107	5	-	-
Control L163	D110	7.5	-	-

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