TUDelft/19 August 2008

From 2008.igem.org

Revision as of 08:58, 26 August 2008 by Ruudjorna (Talk | contribs)

19th August

Testing the Primers

The PCR products were run on two different 1.5% agarose gels. Also a marker was run on the gel. The expected band sizes are ~550bp for IDI, ~900bp for ISPA and ~1200bp for ATOB. It is known that bands end up a little higher when running the SybrSafe product in the gel. The pictures are shown below:

Gel of IDI and ISPA, click picture to enlarge







STILL NEED TO EDIT/PUT IN THE PICTURES

Live stabs

All the live stabs cultures have grown in 5 ml LB + A. We've made glycerol stocks, plated them and grown them in 50 ml LB medium + A o/n again to perform midiprep tomorrow.

Retrieved from "http://2008.igem.org/TUDelft/19_August_2008"