PCR Screening of L173 transformants

Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Ligation L173

Sample	positive control pUC19	negative control no DNA	ligation control (without insert)	L173 (3:1 ratio)	L173 (4:1 ratio)
Number of colonies	many	1	0	2	0

PCR screening programm

Well n°	1	2	3	4	5	6	7
Sample	1 kb DNA ladder	positive PCR control	negative PCR control	negative transformation control clone	L173.1	L173.2	100 bp DNA ladder
Expected size					1894 bp
Measured size

L177 = d134 + d187 (pFlhB-mRFP LVA+)

L175 = d149 + d° (pFliL - ECFP LVA+)

L176 = d149 + d° (pFliL - ECFP LVA-)