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Analysis
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June
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July
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August
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September
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October
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November
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Glycerol Stocks
1mL of each culture (with 2 clones) has been added to 1mL of 40% glycerol.
For each clone, two glycerol stocks have been done.
Stored at -20°C.
MiniPreps
The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 22th.
Name
Plasmid
Description
MP100
B0034
Srtongest RBS (affinity=1)
MP101
J23101
Stongest constitutive promoter
MP102
J23109
Weak constitutive promoter
MP103
R0079
plas
MP104
R0040
pTet
MP105
S03154
RBS-lasI
MP106
S03879
RBS-TetR
MP107
C0079
LasR LVAtag
MP108
C0179
LasR
MP109
E0030
YFP
MP110
E0040
GFP
MP111
E1010
RFP
MP112
OmpR M57I
MP113
EnvZ I281S
MP114
C0040
Tetracyclin repressor
MP115
pSB1A3
High copy number plasmid
MP122
E0840
GFP tripart
Cultures
2007 strains have been taken and put in 6mL of LB with the adequat antibiotic.
Name
Biobricks
Description
S4
J23100
strong constitutive promoter
S5
B0015
double terminator
S7
B0030
strong RBS : 0,6
S8
E0422
ECFP tripart
S13
I0500
pBAD
S14
J23107
Medium constitutive promoter
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