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Imperial College/15 October 2008
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| - | + | =15 October 2008= | |
===Wet Lab=== | ===Wet Lab=== | ||
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| - | *Today | + | ===Digestion of minipreps=== |
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| + | *Today minipreps were prepared. These were digested and run on a gel to confirm the presence of the correct constructs. The folowing constructs were minipreped: Gene art 14 (pXyl-spoVG) and 5' EpsE integration sites. | ||
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| + | *To test that insert with the correct size is present in the plasmid, the minipreps were digestd with EcoRI and PstI. The image below shows the results: | ||
[[Image:Wednesday15thoct.PNG|center|400px]] | [[Image:Wednesday15thoct.PNG|center|400px]] | ||
| - | *Several of construct 14 | + | *Several of construct 14 appear to have succssfully been ligated. In addition all of the 5'EpsE appear to have worked. |
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| + | {{Imperial/EndPage|Notebook|Notebook}} | ||
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| + | *To construct 5'EpsE integration site we used a PCR product and as a result of the approach, there is a possibility that the orientation of this biobrick in the vector is incorrect. Digestion with XbaI SpeI will confirm correct orientation. | ||
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| + | [[Image:Wednesday15thoct2.PNG|center|400px]] | ||
| - | * | + | *If the PCR product has the correct orientation then the insert will fall out with this digestion. Clearly only 5'EpsE-1 is correct. |
Latest revision as of 20:51, 28 October 2008
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15 October 2008Wet LabDigestion of minipreps
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