Imperial College/15 October 2008
From 2008.igem.org
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*Several of construct 14 appear to have succssfully been ligated. In addition all of the 5'EpsE appear to have worked. | *Several of construct 14 appear to have succssfully been ligated. In addition all of the 5'EpsE appear to have worked. | ||
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*To construct 5'EpsE integration site we used a PCR product and as a result of the approach, there is a possibility that the orientation of this biobrick in the vector is incorrect. Digestion with XbaI SpeI will confirm correct orientation. | *To construct 5'EpsE integration site we used a PCR product and as a result of the approach, there is a possibility that the orientation of this biobrick in the vector is incorrect. Digestion with XbaI SpeI will confirm correct orientation. |
Latest revision as of 20:51, 28 October 2008
15 October 2008Wet LabDigestion of minipreps
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