Today mini preps were prepared. These were digested and run on a gel to confirm the presence of the correct constructs. The folowing constructs were mini preped; Gene art 14 (pXyl-spoVG) and 5' EpsE integration sites. To test the correct size insert is present the mini's were digestd with EcoRI and PstI. The image below shows the results:
Several of construct 14 have appeared to have succssfully been ligated. In addition all of the 5'EpsE appear to have worked.
To construct 5'EpsE integration site we used a PCR product and as a result of the approach there is a possibility that the orientation of this biobrick in the vector is incorrect. Digestion with XbaI SpeI will confirm correct orientation.
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