Imperial College/17 September 2008

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==Wet Lab==
==Wet Lab==
===O.D.<sub>600</sub> vs Colony Forming Units===
===O.D.<sub>600</sub> vs Colony Forming Units===
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*The protocol to obtain the calibration curve of O.D.<sub>600</sub> vs Colony Forming Units was performed today. The protocol can be found on this link [http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols/Growth#Protocol | here]]. The following O.D.<sub>600</sub> and dilutions were used:
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*The protocol to obtain the calibration curve of O.D.<sub>600</sub> vs Colony Forming Units was performed today. The protocol can be found on this link [http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols/Growth#Protocol | here]. The following O.D.<sub>600</sub> and dilutions were used:
**O.D<sub>600</sub> of 0.5 = x10<sup>-6</sup> and x10<sup>-7</sup>
**O.D<sub>600</sub> of 0.5 = x10<sup>-6</sup> and x10<sup>-7</sup>
**O.D<sub>600</sub> of 1 = x10<sup>-6</sup> and x10<sup>-7</sup>
**O.D<sub>600</sub> of 1 = x10<sup>-6</sup> and x10<sup>-7</sup>

Revision as of 21:57, 21 September 2008

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17 September 2008

Wet Lab

O.D.600 vs Colony Forming Units

  • The protocol to obtain the calibration curve of O.D.600 vs Colony Forming Units was performed today. The protocol can be found on this link | here. The following O.D.600 and dilutions were used:
    • O.D600 of 0.5 = x10-6 and x10-7
    • O.D600 of 1 = x10-6 and x10-7
    • O.D600 of 1.5 = x10-6 and x10-7
    • O.D600 of 2 = x10-6 and x10-7
    • O.D600 of 2.5 = x10-6 and x10-7
    • O.D600 of 3 = x10-7 and x10-8