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Imperial College/5 September 2008
From 2008.igem.org
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In our second trial run to obtain growth and calibration curves for "B. subtilis", we cultured our "B. subtilis" for two days and continously monitored the changes in OD600. By plotting OD600 against Time/h, we obtained the following growth curve for "B. subtilis". | In our second trial run to obtain growth and calibration curves for "B. subtilis", we cultured our "B. subtilis" for two days and continously monitored the changes in OD600. By plotting OD600 against Time/h, we obtained the following growth curve for "B. subtilis". | ||
| - | [[Image:Trial Run Growth Curve. | + | [[Image:Trial Run Growth Curve.PNG|thumb|600px|center|Growth Curve for "B. subtilis"]] |
===Calibration Curve=== | ===Calibration Curve=== | ||
Revision as of 15:35, 5 September 2008
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5 September 2008WetlabColony CountingAfter re-calibrating our illuminator, it can now count colonies with a fair degree of accuracy. Manual addition and removal of points allows us to fine-tune the results, so we will use this method from now on.
Trial Run: "B. subtilis" Growth CurveIn our second trial run to obtain growth and calibration curves for "B. subtilis", we cultured our "B. subtilis" for two days and continously monitored the changes in OD600. By plotting OD600 against Time/h, we obtained the following growth curve for "B. subtilis". File:Trial Run Growth Curve.PNG Growth Curve for "B. subtilis" Calibration CurveSuggestions for Further Improvements
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