Team:Paris/August 17

From 2008.igem.org

(Difference between revisions)
(→Analysis of the transformation we did yesterday)
(→Evaluation of the number of colonies)
Line 91: Line 91:
=='''Analysis of the other transformation we did [[Team:Paris/August 16 |yesterday]]'''==
=='''Analysis of the other transformation we did [[Team:Paris/August 16 |yesterday]]'''==
===Evaluation of the number of colonies===
===Evaluation of the number of colonies===
 +
 +
{| style="text-align: center;"  Border="2"
 +
|'''Ligation name'''
 +
|'''Number of colonies '''
 +
|'''Autoligation control'''
 +
|-
 +
|L 143 (Kan)
 +
|0
 +
|0
 +
|-
 +
|L 144 (Kan)
 +
|0
 +
|0
 +
|-
 +
|L 145 (Amp)
 +
|452
 +
|430
 +
|-
 +
|L 146 (Amp)
 +
|~ 2000
 +
|430
 +
|-
 +
|L 147 (Amp)
 +
|~3500
 +
|>5000
 +
|}
 +
===PCR Screening===
===PCR Screening===
===Minipreps===
===Minipreps===
=='''Kok-Phen transformation we did [[Team:Paris/August 16 |yesterday]]'''==
=='''Kok-Phen transformation we did [[Team:Paris/August 16 |yesterday]]'''==

Revision as of 15:53, 17 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Contents

Analysis of the transformation we did yesterday

Number of colonies and flurorescence

Ligation name Description Antibio Number Colonies observed Fluorescence Comments
Ligations
L150 D105(BV) - D146(BI)
pLas - rbs-TetR-GFP Tripart 		Amp 3 Yes OK
L151 D147(FI) - D125(FV)
rbs-LasR - Double terminator 		Kan 21 No OK
Controls
C1 D105(BV) Amp 150 NO OK
C2 D125(FV) Kan 2 No OK
Positive Control pUC19 Amp 416 (efficiency 4.10^7) No OK

PCR Screening

==> Protocol

Minipreps

Miniprep Name Ligation name Antibio Biobricks Description
MP161.1 L150.1 Amp Part icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - rbs-TetR-GFP tripart
MP161.2 L150.2
MP161.3 L150.3
MP162.1 L151.1 Kan Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-LasR - Double terminator
MP162.2 L151.2
MP162.3 L152.3

Analysis of the other transformation we did yesterday

Evaluation of the number of colonies

Ligation name Number of colonies Autoligation control
L 143 (Kan) 0 0
L 144 (Kan) 0 0
L 145 (Amp) 452 430
L 146 (Amp) ~ 2000 430
L 147 (Amp) ~3500 >5000

PCR Screening

Minipreps

Kok-Phen transformation we did yesterday
Retrieved from "http://2008.igem.org/Team:Paris/August_17"