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Team:Paris/August 17
From 2008.igem.org
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Remarks: | Remarks: | ||
| - | L143 and L144 did not work once again. Maybe there is a problem with the digestion, because the primers used present only | + | L143 and L144 did not work once again. Maybe there is a problem with the digestion, because the primers used present only |
| - | nucleotides after the restriction sites. what we should do is cutting pfliL with xbaI and SpeI. As the vector will autoligate, | + | two nucleotides after the restriction sites. what we should do is cutting pfliL with xbaI and SpeI. As the vector will autoligate, |
we should use a phosphatase to prevent this phenomenon. | we should use a phosphatase to prevent this phenomenon. | ||
For L147 and its control (T4), we do not observe any red fluorescence. | For L147 and its control (T4), we do not observe any red fluorescence. | ||
Revision as of 16:00, 17 August 2008
Analysis of the transformation we did yesterday
PCR Screening==> Protocol Minipreps
Analysis of the other transformation we did yesterdayEvaluation of the number of colonies
Remarks:
L143 and L144 did not work once again. Maybe there is a problem with the digestion, because the primers used present only
two nucleotides after the restriction sites. what we should do is cutting pfliL with xbaI and SpeI. As the vector will autoligate,
we should use a phosphatase to prevent this phenomenon.
For L147 and its control (T4), we do not observe any red fluorescence.
It means that the digestion work well, because if it was not digested, the strong promoter J23100 should express mRFP!
We will do the screening anyway.
PCR ScreeningMiniprepsKok-Phen transformation we did yesterday |
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