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Team:Paris/August 18
From 2008.igem.org
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(→Digestion of MP162 (rbs-LasR - Dble ter)) |
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*0,5 µL O19 | *0,5 µL O19 | ||
*12 µL water | *12 µL water | ||
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| + | ==Minipreps & Stocks== | ||
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We wanted to analyse the transformants of the ligations L133 (OmpR* in pSB1A2) and L134 (EnvZ* in pSB1A2) from the experience of august 9th/11th. | We wanted to analyse the transformants of the ligations L133 (OmpR* in pSB1A2) and L134 (EnvZ* in pSB1A2) from the experience of august 9th/11th. | ||
<br>3 clones of L133 and 3 clones of L134 were analysed. | <br>3 clones of L133 and 3 clones of L134 were analysed. | ||
<br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | <br>Each clone tested was then cultured in 7,5 mL LB + ampicilline. | ||
| - | + | =Construction of rbs-LasR-dble ter= | |
| - | == | + | ==Miniprep and stock glycerol of L151 (rbs-LasR - Double terminator)== |
*[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] | *[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] | ||
| + | *[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]] | ||
{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
| + | |'''Miniprep number''' | ||
|'''Stock number''' | |'''Stock number''' | ||
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|'''Ligation name''' | |'''Ligation name''' | ||
|'''Antibio''' | |'''Antibio''' | ||
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|- | |- | ||
|MP162.1 | |MP162.1 | ||
| + | |S162.1 | ||
|L151.1 | |L151.1 | ||
|rowspan="3" |Kan | |rowspan="3" |Kan | ||
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|- | |- | ||
|MP162.2 | |MP162.2 | ||
| + | |S162.2 | ||
|L151.2 | |L151.2 | ||
|- | |- | ||
|MP162.3 | |MP162.3 | ||
| + | |S162.3 | ||
|L151.3 | |L151.3 | ||
|} | |} | ||
=Construction of : promotor-rbs-LasR - Dble ter= | =Construction of : promotor-rbs-LasR - Dble ter= | ||
| - | =='''Digestion | + | =='''Digestion'''== |
==='''Measurement of the concentration of MPs 162'''=== | ==='''Measurement of the concentration of MPs 162'''=== | ||
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[[Image:KR000185b.jpg| thumb|Gel Extraction of D165]] | [[Image:KR000185b.jpg| thumb|Gel Extraction of D165]] | ||
[[Team:Paris/Notebook/Protocols#Migration after extraction |Protocol]] | [[Team:Paris/Notebook/Protocols#Migration after extraction |Protocol]] | ||
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{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
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| - | = | + | =Culture of stable strains with biobricks 2008= |
[[Team:Paris/Notebook/Protocols#culture of stable strain with biobrocks 2008 |Protocol]] | [[Team:Paris/Notebook/Protocols#culture of stable strain with biobrocks 2008 |Protocol]] | ||
Latest revision as of 19:50, 4 September 2008
Analysis of the cloning of OmpR* and EnvZ*PCR screening
Reaction mixture (25 µL total volume)
Minipreps & StocksWe wanted to analyse the transformants of the ligations L133 (OmpR* in pSB1A2) and L134 (EnvZ* in pSB1A2) from the experience of august 9th/11th.
Construction of rbs-LasR-dble terMiniprep and stock glycerol of L151 (rbs-LasR - Double terminator)
Construction of : promotor-rbs-LasR - Dble terDigestionMeasurement of the concentration of MPs 162
Digestion
Gel Extraction
Ligation
Culture of stable strains with biobricks 2008
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