Team:Paris/August 2

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(Difference between revisions)

Revision as of 17:34, 4 August 2008

Results of the transformation

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)				To do again
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)				To do again
L102	Strong rbs - YFP D129 (BV) - D118 (BI)				To do again
L103	Strong rbs - GFP D129 (BV) - D122 (BI)				To do again
L104	Strong rbs - lasR activator D129 (BV) - D114 (BI)				To do again
L105	Strong promoter - ECFP D123 (BV) - D130 (BI)				OK
L106	Strong promoter - gfp Tripart D123 (BV) - D131 (BI)				OK
L107	Strongest promoter - ECFP D103 (BV) - D131 (BI)				To do again
L108 n°2 (the right one)	Strong promoter - gfp Tripart D103 (BV) - D130 (BI)				OK
L110	Medium promoter - gfp Tripart D124 (BV) - D131 (BI)				OK
L111	Weak promoter - ECFP D104 (BV) - D130 (BI)				To do again
L112	Weak promoter - gfp D104 (BV) - D131 (BI)				To do again
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)				To do again
L114	AracpBAD - gfp tripart D126 (BV) - D131 (BI)				To do again
L115	pLas - ECFP D105 (BV) - D130 (BI)				To do again
L116	pLas - gfp Tripart D105 (BV) - D131 (BI)				To do again
L117	yfp - Double Terminator D125 (FV) - D117 (FI)				To do again
L118	rfp - Double Terminator D125 (FV) - D121 (FI)				To do again
L119	lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI)				To do again
L120	tetR repressible - ECFP D106 (BV) - D130 (BI)				To do again
L121	Strong promoter - gfp tripart D106 (BV) - D130 (BI)				OK
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)				To do again
L123	RBS lasI - ecfp D107 (BV) - D131 (BI)				To do again
L124	Strongest RBS - rfp D102 (BV) - D122 (BI)				To do again
L125	Strongest RBS - yfp D102 (BV) - D118 (BI)				To do again	L126	Strongest RBS (1)- LacR activator (+LVA) D102 (BV) - D118 (BI)	To do again

Results of the transformation (bis-the good one to leave in august 2)

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)	Amp	608		To do again
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)	Amp	> 4000	GFP positive	OK
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)	Kana	9		To do again
L114	AracpBAD - gfp tripart D126 (BV) - D131 (BI)	Kana	9		To do again
L120	tetR repressible promoter - ECFP D106 (BV) - D130 (BI)	Amp	74		To do again
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)	Amp	4		To do again
L123	RBS lasI - ecfp D107 (BV) - D131 (BI)	Amp	> 4000		To do again
L126	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)	Amp	944		To do again

@@ Line 258: / Line 258: @@
 rbs TetR - ECFP
 <br>D110 (BV) -  D130 (BI)
-|align="center"|
+|align="center"|Amp
 |align="center"|608
 |align="center"|
@@ Line 267: / Line 267: @@
 rbs TetR - GFP tripart
 <br>D110 (BV) -  D131 (BI)
-|align="center"|
+|align="center"|Amp
 |align="center"|> 4000
 |align="center"|GFP positive
@@ Line 276: / Line 276: @@
 AracpBAD - ecfp
 <br>D126 (BV) - D130 (BI)
-|align="center"|
+|align="center"|Kana
 |align="center"|9
 |align="center"|
@@ Line 285: / Line 285: @@
 AracpBAD - gfp tripart
 <br>D126 (BV) - D131 (BI)
-|align="center"|
+|align="center"|Kana
 |align="center"|9
 |align="center"|
@@ Line 294: / Line 294: @@
 tetR repressible promoter - ECFP
 <br>D106 (BV) - D130 (BI)
-|align="center"|
+|align="center"|Amp
 |align="center"|74
 |align="center"|
@@ Line 303: / Line 303: @@
 RBS-lasI - ecfp
 <br>D107 (BV) - D130 (BI)
-|align="center"|
+|align="center"|Amp
 |align="center"|4
 |align="center"|
@@ Line 312: / Line 312: @@
 RBS lasI - ecfp
 <br>D107 (BV) - D131 (BI)
-|align="center"|
+|align="center"|Amp
 |align="center"|> 4000
 |align="center"|
@@ Line 321: / Line 321: @@
 Strongest RBS (1)- LasR activator (+LVA)
 <br>D102 (BV) - D114 (BI)
-|align="center"|
+|align="center"|Amp
 |align="center"|944
 |align="center"|
 |align="center"|To do again
 |}