Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)
(→Results of the transformation (bis-the good one to leave in august 2))
(→Results of the transformation)
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<br>D110 (BV) -  D130 (BI)   
<br>D110 (BV) -  D130 (BI)   
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|0
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 24: Line 24:
<br>D110 (BV) -  D131 (BI)   
<br>D110 (BV) -  D131 (BI)   
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|(1)
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 32: Line 32:
<br>D129 (BV) -  D118 (BI)   
<br>D129 (BV) -  D118 (BI)   
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|+/- 100
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 40: Line 40:
<br>D129 (BV) -  D122 (BI)   
<br>D129 (BV) -  D122 (BI)   
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 48: Line 48:
<br>D129 (BV) -  D114 (BI)   
<br>D129 (BV) -  D114 (BI)   
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 57: Line 57:
<br>D123 (BV) - D130 (BI)
<br>D123 (BV) - D130 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
Line 66: Line 66:
<br>D123 (BV) - D131 (BI)
<br>D123 (BV) - D131 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
Line 74: Line 74:
Strongest promoter - ECFP
Strongest promoter - ECFP
<br>D103 (BV) - D131 (BI)
<br>D103 (BV) - D131 (BI)
-
|align="center"|
+
|align="center"|  
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 84: Line 84:
<br>D103 (BV) - D130 (BI)
<br>D103 (BV) - D130 (BI)
|align="center"|
|align="center"|
 +
|align="center"|+/- 100
|align="center"|
|align="center"|
 +
|align="center"|OK
 +
|-
 +
|align="center"|L109 to complete
 +
|align="center"|
 +
Strong promoter -  ecfp
 +
<br>D124 (BV) - D130 (BI)
 +
|align="center"|
 +
|align="center"|+/- 100
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
Line 93: Line 102:
<br>D124 (BV) - D131 (BI)
<br>D124 (BV) - D131 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
Line 102: Line 111:
<br>D104 (BV) - D130 (BI)  
<br>D104 (BV) - D130 (BI)  
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D104 (BV) - D131 (BI)
<br>D104 (BV) - D131 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D126 (BV) - D130 (BI)
<br>D126 (BV) - D130 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|1
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D126 (BV) - D131 (BI)
<br>D126 (BV) - D131 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|2
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D105 (BV) - D130 (BI)
<br>D105 (BV) - D130 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D105 (BV) - D131 (BI)
<br>D105 (BV) - D131 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D125 (FV) - D117 (FI)
<br>D125 (FV) - D117 (FI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|+/- 30
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D125 (FV) - D121 (FI)
<br>D125 (FV) - D121 (FI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|+/- 30
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D125 (FV) - D113 (FI)
<br>D125 (FV) - D113 (FI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|+/- 30
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 183: Line 192:
<br>D106 (BV) - D130 (BI)
<br>D106 (BV) - D130 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D106 (BV) - D130 (BI)
<br>D106 (BV) - D130 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|OK
|align="center"|OK
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<br>D107 (BV) - D130 (BI)
<br>D107 (BV) - D130 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|0
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
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<br>D107 (BV) - D131 (BI)
<br>D107 (BV) - D131 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|1
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 219: Line 228:
<br>D102 (BV) - D122 (BI)
<br>D102 (BV) - D122 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
Line 228: Line 237:
<br>D102 (BV) - D118 (BI)
<br>D102 (BV) - D118 (BI)
|align="center"|
|align="center"|
-
|align="center"|
+
|align="center"|a lot ++
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
-
 
+
|-
|align="center"|L126
|align="center"|L126
|align="center"|
|align="center"|
Line 237: Line 246:
<br>D102 (BV) - D118 (BI)
<br>D102 (BV) - D118 (BI)
|align="center"|
|align="center"|
 +
|align="center"|no dish found!!!
 +
|align="center"|
 +
|align="center"|To do again
 +
|-
 +
|align="center"|L127
 +
|align="center"|
 +
gfp (1)- Double terminator
 +
<br>D119 (BV) - D125 (BI)
|align="center"|
|align="center"|
 +
|align="center"|no dish found!!!
|align="center"|
|align="center"|
|align="center"|To do again
|align="center"|To do again
|}
|}
-
 
=== Results of the transformation (bis-the good one to leave in august 2)===
=== Results of the transformation (bis-the good one to leave in august 2)===

Revision as of 17:46, 4 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100 rbs TetR - ECFP


D110 (BV) - D130 (BI)

0 To do again
L101 rbs TetR - GFP tripart


D110 (BV) - D131 (BI)

(1) To do again
L102 Strong rbs - YFP


D129 (BV) - D118 (BI)

+/- 100 To do again
L103 Strong rbs - GFP


D129 (BV) - D122 (BI)

a lot ++ To do again
L104 Strong rbs - lasR activator


D129 (BV) - D114 (BI)

a lot ++ To do again
L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

a lot ++ OK
L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

a lot ++ OK
L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

a lot ++ To do again
L108 n°2 (the right one)

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

+/- 100 OK
L109 to complete

Strong promoter - ecfp
D124 (BV) - D130 (BI)

+/- 100 OK
L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

a lot ++ OK
L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

a lot ++ To do again
L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

a lot ++ To do again
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

1 To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

2 To do again
L115

pLas - ECFP
D105 (BV) - D130 (BI)

a lot ++ To do again
L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

a lot ++ To do again
L117

yfp - Double Terminator
D125 (FV) - D117 (FI)

+/- 30 To do again
L118

rfp - Double Terminator
D125 (FV) - D121 (FI)

+/- 30 To do again
L119

lasR activator + LVA - Double Terminator
D125 (FV) - D113 (FI)

+/- 30 To do again
L120

tetR repressible - ECFP
D106 (BV) - D130 (BI)

a lot ++ To do again
L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

a lot ++ OK
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

0 To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

1 To do again
L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

a lot ++ To do again
L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)

a lot ++ To do again
L126

Strongest RBS (1)- LacR activator (+LVA)
D102 (BV) - D118 (BI)

no dish found!!! To do again
L127

gfp (1)- Double terminator
D119 (BV) - D125 (BI)

no dish found!!! To do again

Results of the transformation (bis-the good one to leave in august 2)

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100

rbs TetR - ECFP
D110 (BV) - D130 (BI)

Amp 608 To do again
L101

rbs TetR - GFP tripart
D110 (BV) - D131 (BI)

Amp > 4000 GFP positive OK
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

Kana 9 To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

Kana 9 To do again
L120

tetR repressible promoter - ECFP
D106 (BV) - D130 (BI)

Amp 74 To do again
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

Amp 4 To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

Amp > 4000 To do again
L126

Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)

Amp 944 To do again
Retrieved from "http://2008.igem.org/Team:Paris/August_2"